Baron et al., Science Translational Medicine, 2018. Epigenetic immune cell counting in human blood samples for immunodiagnostics.
Treg Assay - Target Discovery
Floess et al., PloS 2007 5(2):e38. Epigenetic control of the foxp3 locus in regulatory T cells.
Baron et al., Eur J Immunol 2007 37(9): 2378-89. DNA demethylation in the human FOXP3 locus discriminates regulatory T cells from activated FOXP3(+) conventional T cells.
Polansky et al., Eur J Immunol 2008 38(6):1654-63. DNA methylation controls Foxp3 gene expression.
Treg Assay - PCR Format and Uses
Wieczorek et al., Canc Res 2009 69(2):599-608. Quantitative DNA methylation analysis of FOXP3 as a new method for counting regulatory T cells in peripheral blood and solid tissue.
Hoffmann et al, Eur J Immunol 2009 39(4):1088-97. Loss of FOXP3 expression in natural human CD4+CD25+ regulatory T cells upon repetitive in vitro stimulation.
Schaub et al., J Allergy Clin Immunol 2009 123(4):774-82. Maternal farm exposure modulates neonatal immune mechanisms through regulatory T cells.
Loddenkemper et al., Cancer Sci 2009 100(6):1112-7. Regulatory (FOXP3+) T cells as target for immune therapy of cervical intraepithelial neoplasia and cervical cancer.
Di Nunzio et al., Blood 2009 114(19):4138-41. Wild-type FOXP3 is selectively active in CD4+CD25hi regulatory T cells of healthy female carriers of different FOXP3 mutations.
Liu et al., PloS One 2010 5(10):e13267. T Regulatory Cells in Cord blood - FOXP3 demethylation as Reliable Quantitative Marker.
McClymont et al., J Immunol 2011 186(7):3918-26. Plasticity of human regulatory T cells in healthy subjects and patients with type 1 diabetes.
Ukena et al., 2011 Blood 118(13):e82-92. Human regulatory T cells in allogeneic stem cell transplantation.
Bestard et al., 2011 Am J Transplant 11(10):2162-2172. Intragraft Regulatory T Cells in Protocol Biopsies Retain Foxp3 Demethylation and Are Protective Biomarkers for Kidney Graft Outcome.
Ferraro et al., 2011 Diabetes 60(11):2903-13. Expansion of Th17 Cells and Functional Defects in T Regulatory Cells Are Key Features of the Pancreatic Lymph Nodes in Patients With Type 1 Diabetes.
Ukena et al., 2011 Exp Hematol 39(12):1152-60. Isolation strategies of regulatory T cells for clinical trials: Phenotype, function, stability, and expansion capacity.
Passerini et al., 2011 J Allergy Clin Immunol 128(6):1376-1379. Forkhead box protein 3 (FOXP3) mutations lead to increased T(H)17 cell numbers and regulatory T-cell instability.
Barzaghi et al., 2012 J Autoimmun 38(1):49-58. Demethylation analysis of the FOXP3 locus shows quantitative defects of regulatory T cells in IPEX-like syndrome.
Hinz et al., 2012 Allergy 67(3):380-9. Cord blood Tregs with stable FOXP3 are influenced by prenatal environment and associated with atopic dermatitis at the age of one year.
Stauch et al., 2012 Transpl Int 25(3):302-13. Induction of bona fide regulatory T cells after liver transplantation - the potential influence of polyclonal antithymocyte globulin.
Peiseler et al., 2012 J Hepatol 57(1):125-32. FOXP3+ regulatory T cells in autoimmune hepatitis are fully functional and not reduced in frequency.
Taubert et al., 2012 Am J Transplant 12(12):3425-36. Enrichment of Regulatory T Cells in Acutely Rejected Human Liver Allografts.
Nettenstrom et al., 2013 J Immunol Methods 387(1-2):81-88. An optimized multi-parameter flow cytometry protocol for human T regulatory cell analysis on fresh and viably frozen cells, correlation with epigenetic analysis, and comparison of cord and adult blood.
T cell assay
Sehouli et al., Epigenetics 2011 6(2):236-46. Epigenetic quantification of tumor-infiltrating T-lymphocytes.
Steinfelder et al., Blood 2011 117(10):8239-46. Epigenetic modification of the human CCR6 gene is associated with stable CCR6 expression in T cells.
Other relevant publications in the field by Epiontis authors
Baron et al., Epigenetics 2006 1(1):55-60. DNA methylation analysis as a tool for cell typing.
Rapko et al., Tissue Eng. 2007 13(9):2271-80. DNA methylation analysis as novel tool for quality control in regenerative medicine.
Hoffmüller 2007, IDrugs 10(11):787-90. MSC 2007: Adult Mesenchymal Stem Cells in Regenerative Medicine. Developments in stem cell therapeutic research. 27-29 August 2007, Cleveland, OH, USA.
Hoffmüller 2007, IDrugs 10(11):784-6. MSC 2007: Adult Mesenchymal Stem Cells in Regenerative Medicine. MSC discovery, potency and markers. 27-29 August, Cleveland, OH, USA.
Zimmermann et al., Arthritis Rheum. 2008 58(9):2743-53. Correlation of COL10A1 induction during chondrogenesis of mesenchymal stem cells with demethylation of two CpG sites in the COL10A1 promoter.
Niemann et al., Cloning Stem Cells 2010 12(1):33-42. DNA methylation patterns reflect epigenetic reprogramming in bovine embryos.
Türbachova et al., Epigenetics. 2013 Nov; 8(11):1226-35. The cellular ratio of immune tolerance (immunoCRIT) is a definite marker for aggressiveness of solid tumors and may explain tumor dissemination patterns.
Schildknecht et al., PLoS One. 2015 May 12;10(5):e0125587. Simultaneous statistical inference for epigenetoc data.